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Further, fusing highly negatively charged peptide tags to scFvs improves their solubility, possibly by increasing the net negative charge 4,12

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Studies in both laboratory rats and in breast cancer indicate a three-fold increase in total MTX in the tumor in the glutamine receiving group as compared with the control group, and this increase was in both the diglutamated and in the pentaglutamated MTX

This fragment oligomerizes and inserts into the plasma membrane, forming pores that disrupt membrane integrity, increase ion influx, and lead to cell swelling and eventual rupture ( Pyroptosis can be initiated via two pathways: classical and non-classical

Informed consent Since the study does not involve any human participants, informed consent is not applicable for the study